Lactoferrin anticorps

N° du produit ABIN458259

Cet anticorps anti-Lactoferrin Polyclonal Chèvre (ABIN458259) détecte spécifiquement Lactoferrin dans IEP, RID et ODD. L’anticorps est réactif avec des échantillons de Singe.

Aperçu rapide pour Lactoferrin anticorps (ABIN458259)

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivité: Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactoferrin est non-conjugé

Application: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Ouchterlony Double Diffusion (ODD)

Détail du produit anti-Lactoferrin anticorps

Fonction: Goat anti Monkey lactoferrin

Specificité: Precipitating polyclonal Goat antiSerum to Monkey lactoferrin.

Réactivité croisée (Details): The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: In immunoelectrophoresis against monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion Mancini, Ouchterlony)and electroimmunodiffusion (Laurell) for the identification and quantitative determination of lactoferrin in body fluids.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogène: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobal action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Precipitation assays. In immunoelectrophoresis use 2 μL monkey serum against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter center well and 10 μL of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion use 1 % antiserum in the gel.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détails sur Lactoferrin

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Autre désignation: Lactoferrin

Pathways: Transition Metal Ion Homeostasis