Lactoferrin anticorps (Biotin)

N° du produit ABIN458279

L’anticorps anti-Lactoferrin Polyclonal Chèvre est utilisé pour la détection de Lactoferrin dans des échantillons de Singe. Il a été validé pour WB, ELISA, ICC, IHC (p) et DB.

Aperçu rapide pour Lactoferrin anticorps (Biotin) (ABIN458279)

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivité: Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactoferrin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Détail du produit anti-Lactoferrin anticorps (Biotin)

Fonction: Goat anti Monkey lactoferrin, conjugated with Biotin

Specificité: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey lactoferrin.

Réactivité croisée (Details): The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong cross-reaction with lactoferrin in Human milk has been observed.

Attributs du produit: In immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. In immunocytochemical and immunohistochemical techniques for the detection of monkey lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in nonisotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogène: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Détails sur Lactoferrin

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Autre désignation: Lactoferrin

Pathways: Transition Metal Ion Homeostasis