Serum anticorps

N° du produit ABIN458420

L’anticorps anti-Serum Polyclonal Lapin est utilisé pour la détection de Serum dans des échantillons de Souris. Il a été validé pour IEP et ODD.

Aperçu rapide pour Serum anticorps (ABIN458420)

Antigène: Serum

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Détail du produit

Fonction: Rabbit anti Mouse Serum proteins

Specificité: Precipitating polyclonal Rabbit antiSerum to Mouse Serum prote

Réactivité croisée (Details): Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross reactivity. Consequently it is not species-specific.

Attributs du produit: In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 13 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogène: Pooled whole mouse serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure

Isotype: IgG

Information d'application

Indications d'application: Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: Serum

Autre désignation: Serum Proteins