Fibrinogen anticorps

N° du produit ABIN458504

Il existe 2+ publications pour ce produit. L’anticorps anti-Fibrinogen Polyclonal Chèvre est utilisé pour la détection de Fibrinogen dans des échantillons de Souris. Il a été validé pour WB, ELISA, IF, DB et IFA.

Aperçu rapide pour Fibrinogen anticorps (ABIN458504)

Antigène: Fibrinogen

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrinogen est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-Fibrinogen anticorps

Fonction: Goat anti Mouse fibrinogen

Specificité: Purified IgG fraction of polyclonal Goat antiSerum to Mouse fibrinogen

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Purification: Purified IgG fraction from antiserum

Immunogène: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Références pour anticorps anti-Fibrinogen (ABIN458504)

Zang, Gustafsson, Jamalpour, Hong, Genové, Welsh: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." dans: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).

Tugues, Roche, Noguer, Orlova, Bhoi, Padhan, Akerud, Honjo, Selvaraju, Mazzone, Tolmachev, Claesson-Welsh: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." dans: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).

Détails sur Fibrinogen

Antigène: Fibrinogen