Erythrocytes anticorps

Détails pour le produit réf. ABIN458786
Antigène
Reactivité
Mouton
12
10
10
8
4
4
4
3
2
Hôte
Lapin
42
16
Clonalité
Polyclonal
Conjugué
Inconjugué
10
6
4
Application
Screening Assay (ScA)
14
6
5
4
3
3
3
3
2
2
2
2
2
1
1
1
Options
Immunogène Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificité Homologous proteins of different species frequently share antigenic determinants. has not been tested.
Attributs du produit IgG fraction of polyclonal rabbit antiserum to sheep erythrocytes
Purification Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Antigène
Indications d'application To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
Restrictions For Research Use only
Format Lyophilized
Concentration IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x10 8 RBC/ml, not less than 1:3,000
Buffer Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur Without preservative
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.