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BETAHYDROXYSTEROID DEHYDROGENASE anticorps (Biotin) Primary Antibody

Reactivité: Pseudomonas testosteroni IF, ELISA, WB Hôte: Lapin Polyclonal
N° du produit ABIN458821
$408.80
Plus shipping costs $45.00
1 mL
local_shipping Destination: Etats-Unis
Envoi sous 5 à 7 jours ouvrables
  • Antigène
    Reactivité
    Pseudomonas testosteroni
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Conjugué
    Biotin
    Application
    Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to β-hydroxysteroid dehydrogenase from Pseudomonas testosteroni cells
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    beta -Hydroxysteroid dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Antigène
    Autre désignation
    beta-hydroxysteroid dehydrogenase
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.2. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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