Glucuronidase beta anticorps (Glucuronidase, beta)

Details for Product anti-GUSB Antibody No. ABIN458831
Antigène
Reactivité
Escherichia coli (E. coli)
105
33
31
9
4
3
2
2
2
1
1
1
1
1
Hôte
Lapin
111
10
Clonalité
Polyclonal
Conjugué
Cet anticorp Glucuronidase beta est non-conjugé
11
5
5
4
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
96
56
50
19
14
13
7
5
4
4
3
3
2
2
2
2
Options
Immunogène beta -Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificité Cross-reactivities against enzymes of other sources may occur but have not been determined.
Attributs du produit IgG fraction of polyclonal rabbit antiserum to β-glucuronidase from Escherichia coli
Purification The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Antigène
Autre désignation beta-glucuronidase (GUSB Antibody Extrait)
Sujet The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Pathways Glycosaminoglycan Metabolic Process
Indications d'application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Restrictions For Research Use only
Format Lyophilized
Concentration IgG protein concentration 10 mg/ml. No foreign proteins added.
Buffer Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur Without preservative
Stock 4 °C/-20 °C
Stockage commentaire The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Produit citée dans: Mickoleit, Lanzloth, Schüler: "A Versatile Toolkit for Controllable and Highly Selective Multifunctionalization of Bacterial Magnetic Nanoparticles." dans: Small (Weinheim an der Bergstrasse, Germany), pp. e1906922, 2020 (PubMed).