FITC anticorps (Fluorescein Isothiocyanate) (Biotin)

Details for Product anti-FITC Antibody No. ABIN458844
Antigène
Reactivité
Chemical
135
4
4
3
3
Hôte
Souris
98
50
39
15
11
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp FITC est conjugé à/à la Biotin
34
32
27
17
11
5
1
1
1
Application
ELISA, Western Blotting (WB)
147
86
53
30
27
26
20
11
7
6
6
3
2
2
2
2
1
1
1
1
Options
Immunogène Highly purified fluorescein isothiocyanate isomer 1.
Clone NI 239
Isotype IgG1 kappa
Specificité This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Attributs du produit Biotin-conjugated purified monoclonal mouse antibody to fluorescein isothiocyanate isomer 1
Antigène
Autre désignation Fluorescein Isothiocyanate Isomer 1
Classe de substances Chemical
Sujet The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
Indications d'application To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Concentration Physicochemical characteristic IgG concentration is 0.4 mg/ml. No foreign proteins added.
Buffer Biotin conjugated purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.