FITC anticorps (Fluorescein Isothiocyanate)

Details for Product anti-FITC Antibody No. ABIN458849
Antigène
Reactivité
Chemical
135
4
4
3
3
Hôte
Souris
98
50
39
15
11
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp FITC est non-conjugé
34
32
28
17
11
5
1
1
1
Application
ELISA, Western Blotting (WB)
147
86
53
30
27
26
20
11
7
6
6
3
2
2
2
2
1
1
1
1
Options
Immunogène Highly purified fluorescein isothiocyanate isomer 1.
Clone NI 239
Isotype IgG1 kappa
Specificité This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Attributs du produit Monoclonal mouse antibody to fluorescein isothiocyanate isomer 1
Antigène
Autre désignation Fluorescein Isothiocyanate Isomer 1
Classe de substances Chemical
Sujet The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immuno-blotting.
Indications d'application To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Concentration IgG concentration is 1.0 mg/ml. No foreign proteins added.
Buffer Delipidated, heat inactivated, lyophilized, stable whole ascites.
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.