Phosphodiesterase anticorps

Détails pour le produit réf. ABIN458944
Pit viper (Crotalus durissus terrificus)
Immunofluorescence (IF), ELISA, Western Blotting (WB)
Immunogène Phosphodiesterase isolated and purified from Crotalus durissus terrificus venom. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificité Cross-reactivities against enzymes of other sources may occur but have not been determined.
Attributs du produit Affinity purified antibodies of polyclonal rabbit antiserum to phosphodiesterase from Crotalus durissus terrificus venom
Purification Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
Sujet The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Indications d'application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Restrictions For Research Use only
Format Lyophilized
Concentration PAb concentration 1 mg/0.5ml. No foreign proteins added.
Buffer Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
Agent conservateur Without preservative
Stock 4 °C/-20 °C
Stockage commentaire The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.