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Ascorbate Oxidase anticorps (Biotin)

AO Reactivité: Pumpkin ELISA, WB, IF Hôte: Lapin Polyclonal Biotin
N° du produit ABIN459005
  • Antigène Tous les produits Ascorbate Oxidase (AO)
    Ascorbate Oxidase (AO)
    Reactivité
    • 7
    • 2
    Pumpkin
    Hôte
    • 9
    Lapin
    Clonalité
    • 9
    Polyclonal
    Conjugué
    • 5
    • 3
    • 1
    Cet anticorp Ascorbate Oxidase est conjugé à/à la Biotin
    Application
    • 8
    • 8
    • 3
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to ascorbate oxidase from cucurbita species
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    Ascorbate oxidase isolated and purified from cucurbita species. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.6. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène
    Ascorbate Oxidase (AO)
    Autre désignation
    Ascorbate Oxidase (AO Produits)
    Synonymes
    anticorps AO, anticorps ascorbate oxidase, anticorps L-ascorbate oxidase, anticorps AO, anticorps LOC9310099
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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