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Cholesterol Oxidase anticorps (Biotin)

choS Reactivité: Nocardia asteroides ELISA, WB, IF Hôte: Lapin Polyclonal Biotin
N° du produit ABIN459018
  • Antigène Tous les produits Cholesterol Oxidase (choS)
    Cholesterol Oxidase (choS)
    Reactivité
    • 4
    • 4
    • 4
    • 2
    Nocardia asteroides
    Hôte
    • 11
    • 3
    Lapin
    Clonalité
    • 14
    Polyclonal
    Conjugué
    • 5
    • 4
    • 3
    • 2
    Cet anticorp Cholesterol Oxidase est conjugé à/à la Biotin
    Application
    • 14
    • 10
    • 3
    • 3
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to cholesterol oxidase from Nocardia erythropolis
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    Cholesterol oxidase isolated and purified from Nocardia erythropolis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.1. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is rec onstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène
    Cholesterol Oxidase (choS)
    Autre désignation
    Cholesterol Oxidase (choS Produits)
    Synonymes
    anticorps SCD63.13, anticorps cholesterol oxidase, anticorps GMC family oxidoreductase, anticorps Cholesterol oxidase, anticorps choS, anticorps SCO4781, anticorps BTH_I0619, anticorps SARE_RS13770, anticorps Lcho_3191, anticorps Npun_R6526, anticorps Amir_2425, anticorps Caci_5961, anticorps Sros_4246, anticorps Kfla_6484, anticorps Snas_3535, anticorps Weevi_2091
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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