NADH Peroxidase anticorps (Biotin)
Aperçu rapide pour NADH Peroxidase anticorps (Biotin) (ABIN459075)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
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Fonction
- Rabbit anti Streptococcus faecalis NADH peroxidase, conjugated with Biotin
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Specificité
- Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to NADH peroxidase from Streptococcus faecalis
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Réactivité croisée (Details)
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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Attributs du produit
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
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Purification
- Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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Immunogène
- NADH peroxidase isolated and purified from Streptococcus faecalis. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Indications d'application
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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Agent conservateur
- Without preservative
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- NADH Peroxidase
Antigène
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