Nuclease P1 anticorps (Biotin)

N° du produit ABIN459081

Détail du produit anti-Nuclease P1 anticorps (Biotin)

Antigène: Nuclease P1

Reactivité: Penicillium sp.

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nuclease P1 est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificité: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nuclease P1 from Penicillium citrinum

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: Nuclease P1 isolated and purified from Penicillium citrinum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to us e, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day . If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur Nuclease P1

Antigène: Nuclease P1

Abstract: Nuclease P1 Produits

Sujet: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.