L’anticorps Lapin Polyclonal anti-Ribonuclease T1 a été validé pour IF, ELISA et WB. Il convient pour détecter Ribonuclease T1 dans des échantillons de Aspergillus oryzae.
N° du produit ABIN459107
Aperçu rapide pour Ribonuclease T1 anticorps (Biotin) (ABIN459107)
Antigène
Ribonuclease T1
Reactivité
Aspergillus oryzae
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Cet anticorp Ribonuclease T1 est conjugé à/à la Biotin
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
Specificité
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Attributs du produit
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to ribonuclease T1 from Aspergillus oryzae
Purification
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Immunogène
Ribonuclease T1 isolated and purified from Aspergillus oryzae. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Reactivité: Aspergillus oryzae
IF, ELISA, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
Restrictions
For Research Use only
Format
Lyophilized
Concentration
IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.1. No foreign proteins added.
Buffer
Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur
Without preservative
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prio r to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the s ame day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Antigène
Ribonuclease T1
Sujet
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.