Enolase anticorps (Biotin)

N° du produit ABIN459122

L’anticorps Mouton Polyclonal anti-Enolase a été validé pour ELISA, WB et IF. Il convient pour détecter Enolase dans des échantillons de Lapin.

Aperçu rapide pour Enolase anticorps (Biotin) (ABIN459122)

Antigène: Enolase

Reactivité: Lapin

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Enolase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-Enolase anticorps (Biotin)

Specificité: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to enolase from rabbit muscle

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: Enolase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.7. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur Enolase

Antigène: Enolase

Sujet: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.