Cellulase anticorps
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- Antigène Tous les produits Cellulase
- Cellulase
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Reactivité
- Trichoderma viride
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Cellulase est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificité
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Attributs du produit
- IgG fraction of polyclonal rabbit antiserum to cellulase from Trichoma viride
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogène
- Cellulase isolated and purified from Trichoma viride. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Indications d'application
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Antigène
- Cellulase
- Autre désignation
- Cellulase (Cellulase Produits)
- Synonymes
- anticorps endo-1,4-beta-glucanase, anticorps cellulase, anticorps Cellulase, anticorps cellulase, anticorps Sros_8280, anticorps Toce_1166, anticorps Acear_1293, anticorps Weevi_1533, anticorps Marky_0330, anticorps Desac_1117, anticorps Halhy_3666
- Sujet
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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