Pyrophosphatase (Inorganic) 1 (PPA1) anticorps

N° du produit ABIN459215

Il existe 1 publication pour ce produit. L’anticorps anti-Pyrophosphatase (Inorganic) 1 Polyclonal Lapin est utilisé pour la détection de Pyrophosphatase (Inorganic) 1 dans des échantillons de Saccharomyces cerevisiae. Il a été validé pour WB, ELISA, IF et DB.

Aperçu rapide pour Pyrophosphatase (Inorganic) 1 (PPA1) anticorps (ABIN459215)

Antigène: Pyrophosphatase (Inorganic) 1 (PPA1)

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)

Détail du produit

Fonction: Rabbit anti Saccharomyces cerevisiae Inorganic pyrophosphatase

Specificité: IgG fraction of polyclonal rabbit antiserum to inorganic pyrophosphatase from yeast.

Réactivité croisée (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.

Purification: Purified IgG fraction from antiserum

Immunogène: Inorganic pyrophosphatase is isolated and purified from yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Indirect immunofluoresence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Référence

Patir-Nebioglu, Andrés, Krebs, Fink, Drzewicka, Stankovic-Valentin, Segami, Schuck, Büttner, Hell, Maeshima, Melchior, Schumacher: "Pyrophosphate modulates plant stress responses via SUMOylation." dans: eLife, Vol. 8, (2019) (PubMed).

Détail du antigène

Antigène: Pyrophosphatase (Inorganic) 1 (PPA1)

Autre désignation: Inorganic Pyrophosphatase

UniProt: P00817