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L-Alanine Dehydrogenase anticorps

Reactivité: Bacillus subtilis ELISA, WB, IF Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN459218
  • Antigène
    L-Alanine Dehydrogenase
    Reactivité
    Bacillus subtilis
    Hôte
    • 3
    Lapin
    Clonalité
    • 3
    Polyclonal
    Conjugué
    • 2
    • 1
    Inconjugué
    Application
    • 3
    • 3
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    IgG fraction of polyclonal rabbit antiserum to L-alanine dehydrogenase from Bacillus subtilis.
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    L-Alanine dehydrogenase isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. No foreign proteins added.
    Buffer
    Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène
    L-Alanine Dehydrogenase
    Synonymes
    anticorps SCI51.13c, anticorps CMS2925, anticorps L-alanine dehydrogenase, anticorps l-alanine dehydrogenase, anticorps SCO1773, anticorps Mmcs_2104, anticorps Mmar10_1058, anticorps Mmc1_0055, anticorps Acel_1288, anticorps Noca_2488, anticorps ald, anticorps Toce_1453, anticorps Ilyop_0407, anticorps Palpr_1204, anticorps Ftrac_3618, anticorps Ocepr_1994, anticorps Sulku_1314, anticorps Tmar_1146, anticorps Intca_1842, anticorps Bache_1992, anticorps Isop_1447, anticorps Despr_2708, anticorps Plabr_0310
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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