SRSF1 anticorps (N-Term)

N° du produit ABIN461956

Cet anticorps Souris Monoclonal détecte spécifiquement SRSF1 dans WB, IHC, IF et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SRSF1 anticorps (N-Term) (ABIN461956)

Antigène: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SRSF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SRSF1 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100 % identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.

Purification: Protein A purified

Immunogène: Recombinant SF2/ASF protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL. A band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur SRSF1

Antigène: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Autre désignation: SFRS1 / SF2

Sujet: Name/Gene ID: SRSF1

Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a

ID gène: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization