BNIP3 anticorps (AA 112-124)

N° du produit ABIN461995

Cet anticorps Souris Monoclonal détecte spécifiquement BNIP3 dans WB, IHC, ELISA, IP et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour BNIP3 anticorps (AA 112-124) (ABIN461995)

Antigène: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BNIP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BNIP3 anticorps

Épitope: AA 112-124

Marque: IHC-plus™

Specificité: Species cross-reactivity: Human.

Purification: Immunoaffinity purified

Immunogène: Recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 μg/mL. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: 4°C while lyophilized, once reconstituted, aliquot and store at -70°C.

Détails sur BNIP3

Antigène: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Autre désignation: NIP3 / BNIP3

Sujet: Name/Gene ID: BNIP3

Synonyms: BNIP3, NIP3

ID gène: 664

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation