RAB3A anticorps (AA 202-217)

N° du produit ABIN462094

Cet anticorps Lapin Polyclonal détecte spécifiquement RAB3A dans WB, IHC et IHC (p). Il présente une réactivité envers Humain et Singe.

Aperçu rapide pour RAB3A anticorps (AA 202-217) (ABIN462094)

Antigène: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAB3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-RAB3A anticorps

Épitope: AA 202-217

Marque: IHC-plus™

Specificité: Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Purification: Immunoaffinity purified

Immunogène: A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2 (25  mM sodium borate, 100  mM boric acid, 75  mM NaCl, 5  mM EDTA (disodium salt)

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur RAB3A

Antigène: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Autre désignation: RAB3A

Sujet: Name/Gene ID: RAB3A
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: RAB3A, RAS-associated protein RAB3A, Ras-related protein Rab-3A

ID gène: 5864

UniProt: P20336

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport