BRCC3 anticorps (AA 1-316)

N° du produit ABIN462274

Cet anticorps Souris Monoclonal détecte spécifiquement BRCC3 dans WB, ELISA, IHC et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour BRCC3 anticorps (AA 1-316) (ABIN462274)

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT3B1

Détail du produit anti-BRCC3 anticorps

Épitope: AA 1-316

Specificité: BRCC36 (1-316aa)

Purification: Protein G purified

Immunogène: Recombinant BRCC36 (1-316aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Détails sur BRCC3

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Autre désignation: BRCC3 / BRCC36

Sujet: Name/Gene ID: BRCC3

Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2

ID gène: 79184

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus