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CRABP1 anticorps (AA 1-137)

Cet anticorps anti-CRABP1 est un anticorps Souris Monoclonal détectant CRABP1 dans WB, ELISA, IHC et IHC (p). Adapté pour Humain et Souris.
N° du produit ABIN462276

Aperçu rapide pour CRABP1 anticorps (AA 1-137) (ABIN462276)

Antigène

Voir toutes CRABP1 Anticorps
CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Reactivité

  • 40
  • 23
  • 2
Humain, Souris

Hôte

  • 36
  • 4
Souris

Clonalité

  • 24
  • 16
Monoclonal

Conjugué

  • 19
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CRABP1 est non-conjugé

Application

  • 35
  • 21
  • 19
  • 12
  • 5
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

AT1A1
  • Épitope

    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    AA 1-137

    Specificité

    Human CRABP1 (1-137aa)

    Purification

    Protein G purified

    Immunogène

    Recombinant human CRABP1 (1-137aa) purified from E. coli

    Type of Immunogen: Recombinant protein

    Isotype

    IgG2b kappa
  • Indications d'application

    Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse eye (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Minimize freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

    Autre désignation

    CRABP1 / CRABP

    Sujet

    Name/Gene ID: CRABP1

    Synonyms: CRABP1, CRABP-I, CRABPI, CRABP

    ID gène

    1381

    UniProt

    P29762
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