CRABP1 anticorps (AA 1-137)
Aperçu rapide pour CRABP1 anticorps (AA 1-137) (ABIN462276)
Antigène
Voir toutes CRABP1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 1-137
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Specificité
- Human CRABP1 (1-137aa)
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Purification
- Protein G purified
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Immunogène
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Recombinant human CRABP1 (1-137aa) purified from E. coli
Type of Immunogen: Recombinant protein -
Isotype
- IgG2b kappa
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Indications d'application
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Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse eye (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.1 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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- CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))
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Autre désignation
- CRABP1 / CRABP
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Sujet
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Name/Gene ID: CRABP1
Synonyms: CRABP1, CRABP-I, CRABPI, CRABP -
ID gène
- 1381
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UniProt
- P29762
Antigène
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