Collagen, Type I (COL1) anticorps

Détails pour le produit réf. ABIN462339
Antigène
Reactivité
Humain
191
83
81
27
13
11
9
9
8
7
7
6
5
5
4
4
4
2
2
2
1
1
Hôte
Lapin
177
64
37
Clonalité
Polyclonal
Conjugué
Inconjugué
28
10
7
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
186
118
88
86
77
75
67
35
26
21
18
15
15
8
6
5
5
4
2
1
1
Options
Marque IHC-plus™
Immunogène Human placental collagen Type I.

Type of Immunogen: Purified protein
Isotype IgG
Specificité Human collagen type. Cross-reactivity: Human collagen type I: 100 % , Human collagen type II: <0.5 %, Human collagen type III: < 25 %, Human collagen type IV: <0.5 %, Human collagen type V: <0.5 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin: <0.5 %.
Purification Ammonium sulfate precipitation
Antigène
Autre désignation Collagen I
Indications d'application Approved: ELISA, IF, IHC, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

Not recommended for: WB
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Lyophilized
Concentration Lot specific
Buffer Lyophilized, PBS,  pH 7.5, 0.1 % Mannitol, 0.1 % Dextran.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C,-20 °C
Stockage commentaire 4°C or -20°C, Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-Collagen, Type I (COL1) antibody (ABIN462339) Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with Collagen I a...
Image no. 2 for anti-Collagen, Type I (COL1) antibody (ABIN462339) Anti-Collagen I antibody IHC of human brain, cerebellum. Immunohistochemistry of form...
Image no. 3 for anti-Collagen, Type I (COL1) antibody (ABIN462339) Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with Collagen I antib...
Image no. 4 for anti-Collagen, Type I (COL1) antibody (ABIN462339) Human Kidney (formalin-fixed, paraffin-embedded) stained with Collagen I antibody ABI...