Collagen Type I anticorps

N° du produit ABIN462339

Détail du produit anti-Collagen Type I anticorps

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Marque: IHC-plus™

Specificité: Human collagen type. Cross-reactivity: Human collagen type I: 100 % , Human collagen type II: <0.5 %, Human collagen type III: < 25 %, Human collagen type IV: <0.5 %, Human collagen type V: <0.5 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin: <0.5 %.

Purification: Ammonium sulfate precipitation

Immunogène: Human placental collagen Type I.

Type of Immunogen: Purified protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IF, IHC, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

Not recommended for: WB

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.5, 0.1 % Mannitol, 0.1 % Dextran.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen I (COL1 Produits)

Synonymes: anticorps collagen type III alpha 1 chain, anticorps COL3A1