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ALPL anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement ALPL dans WB, ELISA, IHC et IHC (p). Il présente une réactivité envers Humain et Boeuf (Vache).
N° du produit ABIN462352

Aperçu rapide pour ALPL anticorps (ABIN462352)

Antigène

Voir toutes ALPL Anticorps
ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivité

  • 86
  • 54
  • 21
  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Boeuf (Vache)

Hôte

  • 87
  • 21
  • 1
  • 1
Souris

Clonalité

  • 86
  • 24
Monoclonal

Conjugué

  • 66
  • 9
  • 7
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp ALPL est non-conjugé

Application

  • 76
  • 38
  • 35
  • 18
  • 17
  • 14
  • 13
  • 13
  • 13
  • 12
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Marque

    IHC-plus™

    Specificité

    Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.

    Purification

    Protein A purified

    Immunogène

    Purified alkaline phosphatase (native) from human bone.

    Type of Immunogen: Purified protein

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 0.09 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

    Autre désignation

    ALPL / Alkaline Phosphatase

    Sujet

    Name/Gene ID: ALPL

    Synonyms: ALPL, Alkaline phosphomonoesterase, AP-TNAP, Alkaline phosphatase liver, APTNAP, Glycerophosphatase, Tissue-nonspecific ALP, TNAP, HOPS, Liver alkaline phosphatase, TNSALP

    ID gène

    249

    UniProt

    P05186
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