MIA anticorps

N° du produit ABIN464851

Détail du produit anti-MIA anticorps

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIA est non-conjugé

Application: ELISA, Western Blotting (WB)

Specificité: Human MIA

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human MIA.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary. ELISA: To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA (LS-C104284) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA. Western Blot: To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur MIA

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA (MIA Produits)

Synonymes: anticorps CD-RAP, anticorps Mia1, anticorps Cdrap, anticorps zgc:194157, anticorps mia, anticorps CDRAP, anticorps Fdp, anticorps MIA, anticorps MIAL, anticorps melanoma inhibitory activity, anticorps otoraplin, anticorps cilia and flagella associated protein 100, anticorps MIA, anticorps Mia, anticorps mia, anticorps Otor, anticorps CFAP100

Sujet: Name/Gene ID: MIA
Family: MIA

Synonyms: MIA, CD-RAP, Melanoma inhibitory activity

ID gène: 8190

UniProt: Q16674