CXCL10 anticorps

N° du produit ABIN465012

L’anticorps Lapin Polyclonal anti-CXCL10 a été validé pour WB, ELISA et Neut. Il convient pour détecter CXCL10 dans des échantillons de Humain.

Aperçu rapide pour CXCL10 anticorps (ABIN465012)

Antigène: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL10 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-CXCL10 anticorps

Specificité: Human IP-10

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IP-10.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IP-10 (100 ng/mL), a concentration of 5-8 μg/mL of this antibody is required. ELISA: To detect hIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IP-10 (LS-C104616) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur CXCL10

Antigène: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Autre désignation: IP-10 / CXCL10

Sujet: Name/Gene ID: CXCL10
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL10, C-X-C motif chemokine 10, Gamma-IP10, IP-10, Gamma IP10, GIP-10, Mob-1, Small-inducible cytokine B10, Crg-2, IFI10, INP10, SCYB10

ID gène: 3627

UniProt: P02778