Interleukin 17a anticorps

N° du produit ABIN465126

Cet anticorps anti-Interleukin 17a est un anticorps Chèvre Polyclonal détectant Interleukin 17a dans ELISA, WB et Neut. Adapté pour Humain.

Aperçu rapide pour Interleukin 17a anticorps (ABIN465126)

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Interleukin 17a est non-conjugé

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Détail du produit anti-Interleukin 17a anticorps

Specificité: Human IL-17A

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IL-17A.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.43 - 0.65 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43-0.65 μg/mL of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Interleukin 17a

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: IL17A / IL17

Sujet: Name/Gene ID: IL17A
Family: Interleukin

Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17

ID gène: 3605

UniProt: Q16552