PF4 anticorps

N° du produit ABIN465152

Détail du produit anti-PF4 anticorps

Antigène: PF4 (Platelet Factor 4 (PF4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PF4 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Human PF-4

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human PF-4.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1 mg/mL-2 mg/mL with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (LS-C104446) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur PF4

Antigène: PF4 (Platelet Factor 4 (PF4))

Autre désignation: CXCL4 / PF4 (PF4 Produits)

Synonymes: anticorps CXCL4, anticorps PF-4, anticorps SCYB4, anticorps Cxcl4, anticorps Scyb4, anticorps Pf4a, anticorps RATPF4A, anticorps PF4A, anticorps CXCL4L1, anticorps CXCL4V1, anticorps PF4-ALT, anticorps SCYB4V1, anticorps PF4, anticorps platelet factor 4, anticorps platelet factor 4 variant 1, anticorps PF4, anticorps Pf4, anticorps LOC703451, anticorps PF4V1, anticorps LOC100524373, anticorps LOC102181854, anticorps LOC100630489

Sujet: Name/Gene ID: PF4
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4

ID gène: 5196

UniProt: P02776