RELM alpha anticorps

N° du produit ABIN465191

L’anticorps anti-RELM alpha Polyclonal Lapin est utilisé pour la détection de RELM alpha dans des échantillons de Souris. Il a été validé pour WB et ELISA.

Aperçu rapide pour RELM alpha anticorps (ABIN465191)

Antigène: RELM alpha

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RELM alpha est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RELM alpha anticorps

Specificité: Mouse RELMalpha

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Murine RELMalpha.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMalpha (LS-C104489) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMalpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur RELM alpha

Antigène: RELM alpha

Autre désignation: Fizz1 / RELM Alpha

Sujet: Name/Gene ID: Retnla

Synonyms: Retnla, Fizz-1, Fizz1, HIMF, RELM Alpha, RELM-alpha, RELMa, RELMalpha, Resistin like alpha, Xcp2

ID gène: 57262

UniProt: Q9EP95