PLGF anticorps (Biotin)
Aperçu rapide pour PLGF anticorps (Biotin) (ABIN465296)
Antigène
Voir toutes PLGF (PGF) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human PlGF-1
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human PlGF-1.
Type of Immunogen: Cell extract
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Indications d'application
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hPlGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PlGF as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. Western Blot: To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile PBS containing 0.1 % BSA
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- PLGF (PGF) (Placenta Growth Factor (PGF))
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Autre désignation
- PGF / PLGF
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Sujet
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Name/Gene ID: PGF
Family: PDGF
Synonyms: PGF, D12S1900, PGFL, PlGF-2, Placental growth factor, Placental growth factor-like, PLGF, Placenta growth factor, SHGC-10760 -
ID gène
- 5228
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UniProt
- P49763
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Pathways
- VEGFR1 Specific Signals
Antigène
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