TNFSF13 anticorps
Aperçu rapide pour TNFSF13 anticorps (ABIN465417)
Antigène
Voir toutes TNFSF13 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human APRIL
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human APRIL.
Type of Immunogen: Cell extract
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Indications d'application
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hAPRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human APRIL (LS-C104484) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hAPRIL. Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
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Autre désignation
- TNFSF13 / APRIL
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Sujet
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Name/Gene ID: TNFSF13
Family: TNF
Synonyms: TNFSF13, APRIL, CD256, CD256 antigen, TALL-2, TALL2, ZTNF2, TNF-related death ligand 1, TRDL-1, UNQ383/PRO715 -
ID gène
- 8741
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UniProt
- O75888
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Antigène
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