RETNLB anticorps

N° du produit ABIN465494

Il existe 2+ publications pour ce produit. L’anticorps anti-RETNLB Polyclonal Lapin est utilisé pour la détection de RETNLB dans des échantillons de Souris. Il a été validé pour WB et ELISA.

Aperçu rapide pour RETNLB anticorps (ABIN465494)

Antigène: RETNLB (Resistin Like beta (RETNLB))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RETNLB est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RETNLB anticorps

Specificité: Mouse RELMbeta

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Murine RELMbeta.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta (LS-C104626) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMbeta. Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Références pour anticorps anti-RETNLB (ABIN465494)

Varyani, Löser, Filbey, Harcus, Drurey, Poveda, Rasid, White, Smyth, Gerbe, Jay, Maizels: "The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF)." dans: Mucosal immunology, (2022) (PubMed).

Gerbe, Sidot, Smyth, Ohmoto, Matsumoto, Dardalhon, Cesses, Garnier, Pouzolles, Brulin, Bruschi, Harcus, Zimmermann, Taylor, Maizels, Jay: "Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites." dans: Nature, Vol. 529, Issue 7585, pp. 226-30, (2016) (PubMed).

Détails sur RETNLB

Antigène: RETNLB (Resistin Like beta (RETNLB))

Autre désignation: FIZZ1 / RELM-Beta

Sujet: Name/Gene ID: RETNLB

Synonyms: RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2

ID gène: 84666

UniProt: Q9BQ08

Pathways: Hormone Activity