Thymic Stromal Lymphopoietin anticorps
Aperçu rapide pour Thymic Stromal Lymphopoietin anticorps (ABIN465621)
Antigène
Voir toutes Thymic Stromal Lymphopoietin (TSLP) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Specificité
- Human TSLP
-
Purification
- Immunoaffinity purified
-
Immunogène
-
E.coli derived recombinant Human TSLP.
Type of Immunogen: Cell extract
-
-
-
-
Indications d'application
-
Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human prostate. The recommended concentration is 0.125 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hTSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP (LS-C104690) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTSLP. Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTSLP is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Commentaires
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Sterile water
-
Concentration
- Lot specific
-
Buffer
- Lyophilized from PBS, pH 7.2
-
Conseil sur la manipulation
- Avoid freeze/thaw cycles.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
-
- Thymic Stromal Lymphopoietin (TSLP)
-
Autre désignation
- TSLP
-
Sujet
-
Name/Gene ID: TSLP
Family: Cytokine
Synonyms: TSLP, Thymic stromal lymphopoietin -
ID gène
- 85480
-
UniProt
- Q969D9
Antigène
-