Nerve Growth Factor anticorps

N° du produit ABIN465644

Détail du produit anti-Nerve Growth Factor anticorps

Antigène: Nerve Growth Factor (NGF)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificité: Human beta-NGF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 8 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF (NGF Produits)

Synonymes: anticorps Beta-NGF, anticorps HSAN5, anticorps NGFB, anticorps Ngfb, anticorps ngfb, anticorps ns:zft386, anticorps xx:zft386, anticorps zgc:109730, anticorps beta-NGF, anticorps hsan5, anticorps beta-ngf, anticorps NGF, anticorps ngf, anticorps nerve growth factor, anticorps nerve growth factor b (beta polypeptide), anticorps nerve growth factor S homeolog, anticorps NGF, anticorps Ngf, anticorps ngfb, anticorps ngf, anticorps ngf.S

Sujet: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

ID gène: 4803

UniProt: P01138

Pathways: Regulation of Cell Size