ICAM1 anticorps

N° du produit ABIN465661

Cet anticorps anti-ICAM1 Polyclonal Lapin (ABIN465661) détecte spécifiquement ICAM1 dans WB et ELISA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour ICAM1 anticorps (ABIN465661)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ICAM1 anticorps

Specificité: Human ICAM-1

Purification: Immunoaffinity purified

Immunogène: CHO cell derived recombinant Human ICAM-1.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal adenocarcinoma (moderately differentiated). The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human ICAM-1 (10 ng/mL), a concentration of 8 u/mL of this antibody is required. ELISA: To detect Human ICAM-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ICAM-1 (LS-C104787) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Western Blot: To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM1 / CD54

Sujet: Name/Gene ID: ICAM1
Family: ICAM

Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

ID gène: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling