Cyclin D1/D2 anticorps

N° du produit ABIN470767

Cet anticorps Souris Monoclonal détecte spécifiquement dans IP, WB, IHC et IHC (p). Il présente une réactivité envers Humain, Hamster, Souris et Rat.

Aperçu rapide pour Cyclin D1/D2 anticorps (ABIN470767)

Antigène: Cyclin D1/D2

Reactivité: Humain, Hamster, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Specificité: Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.

Purification: Protein A purified

Immunogène: Recombinant human Cyclin D1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: IHC, IHC-P, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer,  pH 6.0, microwaved 3-5 minutes, three times).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détail du antigène

Antigène: Cyclin D1/D2

Autre désignation: Cyclin D1,2

Sujet: This antibody detects: Cyclin D1+D2