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GQ alpha/g11 alpha (C-Term) anticorps

Cet anticorps Lapin Polyclonal détecte spécifiquement dans IP et WB. Il présente une réactivité envers Roussette (Chauve-souris), Poulet, Boeuf (Vache), Chien, Chévre, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat, Xenopus laevis et Poisson zèbre (Danio rerio).
N° du produit ABIN470875

Aperçu rapide pour GQ alpha/g11 alpha (C-Term) anticorps (ABIN470875)

Antigène

GQ alpha/g11 alpha

Reactivité

Roussette (Chauve-souris), Poulet, Boeuf (Vache), Chien, Chévre, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat, Xenopus laevis, Poisson zèbre (Danio rerio)

Hôte

Lapin

Clonalité

Polyclonal

Application

Immunoprecipitation (IP), Western Blotting (WB)
  • Épitope

    C-Term

    Specificité

    Specific for human Gq alpha and G11 alpha. Does not recognize other non-pertussis toxin-sensitive G proteins. Species cross-reactivity: Mouse and human.

    Homologie

    Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%) Water flea (90%).

    Purification

    Purified

    Immunogène

    KLH-conjugated, synthetic peptide (QLNLKEYNLV) corresponding to amino acids 350- 359 of G11 alpha or 344-353 of Gq alpha. This sequence is highly conserved among species. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%), Water flea (90%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    Isotype

    IgG
  • Indications d'application

    Approved: IP (1:500 - 1:2000), WB

    Usage: Suitable for use in Western Blot analysis and Immunoprecipitation. Immunoprecipitation: 1:500-1:2000 (detects Gq alpha and G11 alpha in 20 μg of mouse brain microsomes and membranes, detected Gq/11 a in 10 μg of mouse 3T3/NIH fibroblast soluble plasma membranes.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    avoid freeze thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Antigène

    GQ alpha/g11 alpha
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