Soluble Terminal Complement Complex (sC5b-9) anticorps

N° du produit ABIN472099

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, IHC (fro), WB, ELISA et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Soluble Terminal Complement Complex (sC5b-9) anticorps (ABIN472099)

Antigène: Soluble Terminal Complement Complex (sC5b-9)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3R2-0

Détail du produit

Specificité: Recognizes the SC5b-9 complex of around 330 kD. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kD C5b which is bound to cells via 71 kD C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kD S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Removal of Sodium Azide is recommended prior to use in functional assays. Reported to work in western blotting applications.

Purification: Affinity purified

Immunogène:

Type of Immunogen: Native protein

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.4, <0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détail du antigène

Antigène: Soluble Terminal Complement Complex (sC5b-9)