DYNC1I1 anticorps (N-Term)

N° du produit ABIN472619

L’anticorps Souris Monoclonal anti-DYNC1I1 a été validé pour WB, ICC, IF et IP. Il convient pour détecter DYNC1I1 dans des échantillons de Rat, Souris, Boeuf (Vache) et Mouton.

Aperçu rapide pour DYNC1I1 anticorps (N-Term) (ABIN472619)

Antigène: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))

Reactivité: Rat, Souris, Boeuf (Vache), Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYNC1I1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-DYNC1I1 anticorps

Épitope: N-Term

Specificité: Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.

Purification: Purified

Immunogène: Bovine brain cytoplasmic dynein.

Type of Immunogen: Purified protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ICC (1:50 - 1:100), IF, IP, WB (1:1000 - 1:5000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 μL/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur DYNC1I1

Antigène: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))