Proliferation Marker anticorps

N° du produit ABIN478915

Cet anticorps Souris Monoclonal détecte spécifiquement Proliferation Marker dans IHC, WB, ELISA, IHC (fro), IHC (p) et IP. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Proliferation Marker anticorps (ABIN478915)

Antigène: Proliferation Marker

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit

Specificité: Reacts with a 14-16kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat.

Purification: Protein A purified

Immunogène: Spleen cells of a human patient with hairy cell leukemia.

Type of Immunogen: Cells

Isotype: IgM kappa

Information d'application

Indications d'application: Approved: ELISA (1:1000), IHC, IHC-Fr, IHC-P, IP, WB (1:50)

Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry frozen and formalin/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. ELISA: 1:1000. Immunohistochemistry:1:50 to 1:100. Western Blot: 1:50 to 1:100. Positive control: Tonsilar tissue.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détail du antigène

Antigène: Proliferation Marker