Nitrotyrosine anticorps

N° du produit ABIN479556

Cet anticorps anti-Nitrotyrosine est un anticorps Souris Monoclonal détectant Nitrotyrosine dans WB, IP et ICC. Adapté pour .

Aperçu rapide pour Nitrotyrosine anticorps (ABIN479556)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-Nitrotyrosine anticorps

Specificité: Recognizes nitrated proteins from all species.

Purification: Protein G purified

Immunogène: Purified, nitrated Keyhole Limpet Hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype: IgG2b kappa

Information d'application

Indications d'application: Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95 % ethanol/5 % acetic acid.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Classe de substances: Chemical