IFNGR1 anticorps (Extracellular Domain)

N° du produit ABIN480396

Cet anticorps anti-IFNGR1 est un anticorps Chèvre Polyclonal détectant IFNGR1 dans WB, IHC, ELISA, ICC, Neut et Func. Adapté pour Humain.

Aperçu rapide pour IFNGR1 anticorps (Extracellular Domain) (ABIN480396)

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFNGR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Neutralization (Neut), Functional Studies (Func)

Détail du produit anti-IFNGR1 anticorps

Épitope: Extracellular Domain

Specificité: Binds to and neutralizes human Interferon gamma receptor 1, 15.756kD. Blocks cell surface human Interferon gamma receptor 1-mediated bioactivity of recombinant human Interferon gamma receptor 1. In direct ELISA and Western Blot, does not cross-react with human Interferon gamma receptor chain2.

Purification: Immunoaffinity purified

Immunogène: Recombinant, human Interferon gamma receptor chain 1, NS0-derived, extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, Func, ICC, IHC (15 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Neutralization of Human IFN-g R1 Bioactivity: The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is ~0.5-2 μg/mL in the presence of 2 ng/mL of rhIFN-g, using a confluent culture of HeLa cells. ELISA (Direct): 0.5-1 μg/mL. The detection limit for rhIFN-g R1 is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for is ~5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 μg/mL. Detects IFN-g R1 in human PBMC's (peripheral blood mononuclear cells) (fluorescence immunocytochemistry).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 5 % trehalose. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur IFNGR1

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Autre désignation: CD119 / IFNGR1

Sujet: Name/Gene ID: IFNGR1
Family: Interferon Receptor

Synonyms: IFNGR1, AVP, type 2, Antiviral protein, type 2, CD119, CD119 antigen, IFNGR, IFN-gamma receptor 1, IFN-gamma-R1, Immune interferon receptor 1, Interferon gamma receptor 1, CDw119

ID gène: 3459

UniProt: P15260

Pathways: Interferon-gamma Pathway