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CCL4 anticorps

Cet anticorps anti-CCL4 est un anticorps Chèvre Polyclonal détectant CCL4 dans WB, ELISA, IHC et Neut. Adapté pour Humain et Souris.
N° du produit ABIN480888

Aperçu rapide pour CCL4 anticorps (ABIN480888)

Antigène

Voir toutes CCL4 Anticorps
CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité

  • 64
  • 42
  • 20
  • 8
  • 7
  • 7
  • 2
  • 2
  • 1
  • 1
Humain, Souris

Hôte

  • 99
  • 12
  • 2
  • 2
  • 1
  • 1
Chèvre

Clonalité

  • 101
  • 15
Polyclonal

Conjugué

  • 62
  • 14
  • 11
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CCL4 est non-conjugé

Application

  • 95
  • 42
  • 37
  • 31
  • 25
  • 14
  • 13
  • 13
  • 8
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)
  • Specificité

    Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10 % cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5 % cross-reactivity with mouse MIP-1b. <1 % cross-reactivity with other chemokines tested.

    Purification

    Immunoaffinity purified

    Immunogène

    Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).

    Isotype

    IgG
  • Indications d'application

    Approved: ELISA (0.5 - 1 μg/mL), IHC (0.5 - 5 μg/mL), Neut (0.3 - 1.5 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 μg/mL in the presence of 0.04 μg/mL of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 μg/mL in cultured cells or tissue. sections.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 40 % glycerol.

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

    Autre désignation

    CCL4 / SYCA4

    Sujet

    Name/Gene ID: CCL4
    Subfamily: Intercrine beta
    Family: Intercrine

    Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4

    ID gène

    6351

    UniProt

    P13236
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