LY9 anticorps (FITC)
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- Antigène Voir toutes LY9 (CD229) Anticorps
- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp LY9 est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Specificité
- This antibody reacts with Human Ly-9 antigen.
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Attributs du produit
- Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9
- Purification
- Protein-A Sepharose Chromatography.
- Immunogène
- Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte.
- Clone
- HLy9-1-25
- Isotype
- IgG1
- Top Product
- Discover our top product CD229 Anticorps primaire
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- Indications d'application
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Flow Cytometry: 10 μg/mL (final concentration). Positive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% BaN3]2) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD229 monoclonal antibody (10-20 µg/mL) diluted withPBS containing 2% FCS. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytomete.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.05 mg/mL
- Buffer
- PBS, 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
- Autre désignation
- CD229 / LY9 (CD229 Produits)
- Synonymes
- anticorps LY9, anticorps CD229, anticorps SLAMF3, anticorps hly9, anticorps mLY9, anticorps AI893573, anticorps Lgp100, anticorps Ly-9, anticorps T100, anticorps lymphocyte antigen 9, anticorps LY9, anticorps Ly9
- Sujet
- CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9
- ID gène
- 4063
- UniProt
- Q9HBG7
- Pathways
- BCR Signaling
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