CD161 anticorps

N° du produit ABIN487476

Détail du produit anti-CD161 anticorps

Antigène: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD161 est non-conjugé

Application: Flow Cytometry (FACS)

Specificité: This antibody reacts with Human CD161 antigen.

Réactivité croisée (Details): Species reactivity (tested):Human.

Attributs du produit: Synonyms: HNKR-P1a, CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, Naturalkiller cell surface protein P1A, C-type lectin domain family 5 member B

Purification: Protein-A Sepharose Chromatography.

Immunogène: Human polyclonal NK cells. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2 with Balb/cmouse splenocyte.

Clone: HP-3G10

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Lymphocyte cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Flow Cytometric analysis for floating cellsProtocol 1We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Resuspend the cells with washing buffer (5x10e6 cells/mL). 2) Add 50 µL of the cell suspension into each tube, and centrifuge at 500xg for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 3) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well, and incubate for 5 minutes at room temperature(20~25°C). 4) Add 30 µL of the CD161 monoclonal antibody (10-20 µg/mL) diluted with the washingbuffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Add 30 µL of secondary antibody (1: 40 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 7) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 8) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the CD161 monoclonal antibody (50 µg/mL) diluted with the washing bufferinto each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 50 µL of the washing buffer. 7) Add 20 µL of secondary antibody (1: 10 FITC conjugated anti-mouse IgG diluted with thewashing buffer into each tube. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all step described below. 1) Add 20 µL of the CD161 monoclonal antibody (50 µg/mL) diluted with washing buffer[PBS containing 2% fetal calf serum (FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Add 2 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 50 µL of secondary antibody (1: 20 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 5) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective package

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD161

Antigène: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Autre désignation: CD161 / KLRB1 (KLRB1 Produits)

Synonymes: anticorps NKR-P1, anticorps KLRB1, anticorps 4930431A04Rik, anticorps Gm4696, anticorps Klrb1g, anticorps Klrb6, anticorps Ly-55, anticorps Ly55, anticorps NKR-P1G, anticorps Nkrp-1e, anticorps Nkrp1g, anticorps CD161, anticorps CLEC5B, anticorps NKR, anticorps NKR-P1A, anticorps NKRP1A, anticorps hNKR-P1A, anticorps Klrb1d, anticorps Klrb1f, anticorps Nkr-p1e, anticorps killer cell lectin like receptor B1, anticorps killer cell lectin-like receptor subfamily B member 1, anticorps killer cell lectin-like receptor subfamily B, member 1, anticorps KLRB1, anticorps Klrb1

Sujet: CD161, also known as NKR-P1, is a receptor originally identified on the surface of natural killer cells that is also present on some subpopulations of CD8+T cells. CD161 is often used as an NK cell marker since it is present on both immature and mature NK cells. CD161 appears to mediate NK cell activation and is up-regulated by IL-12. CD161 is expressed as a disulfide homodimer of ~60 kDa while the ligand for CD161 appears to be a carbohydrate structure that includes GalNAc and GlcNAc structures. CD161+ T cells are commonly found in human liver and intestinal epithelium where they may play a role in local immunity.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

ID gène: 3820

UniProt: Q12918