Phosphotyrosine anticorps (phosphorylated)

N° du produit ABIN487507

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphotyrosine dans WB, IP, IF et IHC (fro).

Aperçu rapide pour Phosphotyrosine anticorps (phosphorylated) (ABIN487507)

Antigène: Phosphotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: G104

Détail du produit anti-Phosphotyrosine anticorps

Épitope: phosphorylated

Purification: Protein G Chromatography.

Immunogène: Phosphotyrosine, alanine and glyceine in a 1: 1: 1 ratio polymerized in the presence ofKeyhole Limpet Hemocyanin with 1-ethyl-3-(3?-dimentrylaminopropyl) carbodiimide.

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1/1000 (1,2).1 μg/mL of this antibody was sufficient for detection of phosphorylated tyrosine residues in10 μg of Rat tissue lysate by colorimetric Immunoblot analysis using Goat anti-Rat IgG-HRPas the secondary antibody. Immunoprecipitation (9). Immunofluorescence (2). Immunohistochemitry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).