SELPLG anticorps (FITC)

N° du produit ABIN488144

Détail du produit anti-SELPLG anticorps (FITC)

Antigène: SELPLG (Selectin P Ligand (SELPLG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SELPLG est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: This antibody reacts with Human PSGL-1 antigen.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein-A Sepharose Chromatography.

Immunogène: Human Neutrophil PSGL-1. Remarks: This Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte.

Clone: PL2

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: Jurkat, HL60.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet aftertapping. Mix well, and incubate for 5 minutes at roomtemperature (20~25°C). 3) Add 30 µL of the FITC labeled CD162 (PL2) monoclonal antibody (10-20 µg/mL) dilutedwith the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD162 (PL1) monoclonal antibody (50 µg/mL)into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C)5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.05 mg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur SELPLG

Antigène: SELPLG (Selectin P Ligand (SELPLG))

Autre désignation: CD162 / PSGL1 (SELPLG Produits)

Synonymes: anticorps CD162, anticorps CLA, anticorps PSGL-1, anticorps PSGL1, anticorps SELPLG, anticorps SELPG, anticorps Psgl-1, anticorps Psgl1, anticorps Selp1, anticorps Selpl, anticorps selectin P ligand, anticorps selectin, platelet (p-selectin) ligand, anticorps clasper, anticorps SELPLG, anticorps Selplg, anticorps cla

Sujet: CD162 is a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand

ID gène: 6404

NCBI Accession: NP_002997

UniProt: Q14242