Ricin anticorps

N° du produit ABIN488640

Cet anticorps anti-Ricin Monoclonal Souris (ABIN488640) détecte spécifiquement Ricin dans ELISA et IHC. L’anticorps est réactif avec des échantillons de Ricinus communis.

Aperçu rapide pour Ricin anticorps (ABIN488640)

Antigène: Ricin

Reactivité: Ricinus communis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ricin est non-conjugé

Application: ELISA, Immunohistochemistry (IHC)

Détail du produit anti-Ricin anticorps

Fonction: Anti-Ricin (RTA subunit), clone MMA

Réactivité croisée (Details): Ricinus communis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Ricinus communis

Purification: Purified IgG

Immunogène: Ricin

Isotype: IgG1

Information d'application

Indications d'application: The optimal working dilution should be determined by the investigator, for specific application

Commentaires:

Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule. This antibody does not cross-react to whole ricin molecule.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Purified IgG in PBS pH 7.4 with mannitol.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C,-80 °C

Stockage commentaire: Store at -70°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added

Détails sur Ricin

Antigène: Ricin

Sujet:

Background: Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.

Additional information: Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1 % , dextran 1 %, NaCl 100 mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/mL) or by Bradford methodRicin detection limit is 5 ng/mL, signal-to-noise ratio is 15-50 up to 100 ng/mLEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule. Suggested protocol can be found here.

Poids moléculaire: 29.8 kDa